Nanobret Assay In Vivo Cell Based Kd

Microtubule / Tubulin In Vivo Assay Kit

BK038 KIT 30-100 assays
EUR 837.2

Human IgG antibody Laboratories manufactures the nanobret assay in vivo cell based kd reagents distributed by Genprice. The Nanobret Assay In Vivo Cell Based Kd reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact kd assay. Other Nanobret products are available in stock. Specificity: Nanobret Category: Assay Group: In Vivo

EpiQuik In Vivo Protein Sumoylation Assay Ultra Kit (Colorimetric)

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  • 96 Assays
  • 48 Assays
  • 96 Assays 

PDE4B Cell-Based Activity Assay Kit

500 rxns.
EUR 610
Description: Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE4B has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP. Inhibition of PDE4B activity by its inhibitors leads to an elevated intracellular level of cAMP. The PDE4B gene encodes at least 4 different isoforms, and has been linked to inflammation in monocytes by its regulation of the toll-receptor pathway. It is also highly expressed in the central nervous system, and has been targeted as a potential treatment for autism, depression, schizophrenia, and other conditions._x000D_The PDE4B cell-based activity assay kit is designed for screening inhibitors of PDE4B1 in cultured cells. The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter. However, when cells are co-transfected with PDE4B1 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4B inhibitor to inhibit PDE4B1 activity, cAMP level is restored, resulting in higher luciferase activity._x000D_The kit includes CRE luciferase reporter (premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency), PDE4B1 expression vector, and forskolin.

PDE4D Cell-Based Activity Assay Kit

500 rxns.
EUR 645
Description: The PDE4D cell-based activity assay is designed for screening inhibitors of PDE4D7 in cultured cells. The assay is based on transfecting cells with the CRE luciferase reporter. _x000D_CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter. However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity.

PD-1 Cell-Based Assay Medium

100 ml
EUR 120
Description: This liquid Assay Medium is optimized for use with the PD-1 cell-based assay using the BPS Bioscience's PD-1/NFAT Reporter-Jurkat cell line (Cat #60535). _x000D_

PDE1B Cell-Based Reporter Assay Kit

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PDE4D Cell-Based Activity Assay Kit

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LDL Uptake Assay Kit (Cell-Based)

each
EUR 816

In Vivo information

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 3095

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 750

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 3215

CytoSelect™ 48-Well Cell Contraction Assay Kit

CBA-5021 48 assays
EUR 575

Radius 24-Well Cell Migration Assay, (Laminin Coated)

CBA-125-LN 24 assays
EUR 714
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145 384 assays
EUR 1208.4
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145-5 5 x 384 assays
EUR 4681.2
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

CytoSelect™ Cell Proliferation Assay Reagent (Colorimetric)

CBA-253 10 mL
EUR 320

Radius 24-Well Cell Migration Assay, (Fibronectin Coated)

CBA-125-FN 24 assays
EUR 714
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Endothelial Tube Formation Assay (In Vitro Angiogenesis Assay)

CBA-200 50 assays
EUR 470

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size

CBA-135-T 24 assays
EUR 518.4
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

Radius 24-Well Cell Migration Assay, (ECM Array Coated)

CBA-125-ECM 24 wells
EUR 838.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect™ 96-Well Hematopoietic Colony Forming Cell Assay (96 assays)

CBA-320 96 assays
EUR 440

CytoSelect 48-well Cell Adhesion Assay (Laminin, Colorimetric)

CBA-056 48 assays
EUR 512.4
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Laminin.

CytoSelect 48-well Cell Adhesion Assay (Laminin, Fluorometric)

CBA-057 48 assays
EUR 553.2
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Laminin.

CytoSelect 24-well Laminin Cell Invasion Assay, Fluorometric

CBA-111-LN 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric

CBA-112-LN 96 assays
EUR 908.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.